Skin and airway epithelia
ELISA, RIA, IHC not established
(lyophilisate) 1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
A high affinity purification column was use to purify Beta-Defensin-3 (HBD-3/MBD-3) by Nordic MUbio by chromatographic size exclusion.
Mixture of synthetic peptides 15 aa near C-terminus of the mature HBD-3 (LPKEEQIGKCSTRGR) and 13 aa of N-terminal MBD-3, coupled to KLH for immunisation of rabbits
1 of protein A or G purified by Nordic MUbio will give more specificity than crude serum.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.